Nhau - Kuongorora Kwemazinga Akanyungudika Oxygen muBio Pharmaceutical Fermentation Process

Chii chinonzi Dissolved Oxygen?

Yakanyungudutswa Oxygen (DO) inoreva molecular oxygen (O_₂) inonyungudika mumvura. Inosiyana nemaatomu eokisijeni ari mumamorekuru emvura (H_₂O), sezvazviri mumvura muchimiro cheokisijeni mamorekuru akazvimirira, angave anobva mumhepo kana kugadzirwa kuburikidza nephotosynthesis nezvinomera zvemumvura. Iko kusungirirwa kweDO kunokanganiswa nezvinhu zvakasiyana, zvinosanganisira tembiricha, salinity, kuyerera kwemvura, uye zviitiko zvebiology. Saka nekudaro, inoshanda sechiratidzo chakakosha chekuongorora hutano uye kusvibiswa kwenzvimbo dzemvura.

Okisijeni yakanyungudika inoita basa rakakosha mukusimudzira hutachiona hwemetabolism, kukurudzira kufema kweserura, kukura, uye biosynthesis yezvigadzirwa zvemetabolism. Nekudaro, mazinga epamusoro eokisijeni yakanyungudika haasi anobatsira nguva dzose. Okisijeni yakawandisa inogona kutungamira kune mamwe mametabolism ezvigadzirwa zvakaunganidzwa uye zvinogona kukonzera kuita kwehupfu. Iwo akanyanya DO mazinga anosiyana pakati pemhando dzakasiyana dzebhakitiriya. Semuyenzaniso, panguva ye biosynthesis yepenicillin, DO inowanzochengetedzwa paanenge 30% mweya saturation. Kana DO inodonha kusvika zero uye ikaramba iri padanho iroro kwemaminetsi mashanu, kuumbwa kwechigadzirwa kunogona kukanganisa zvakanyanya. Kana chirwere ichi chikaramba chiripo kwemaminetsi makumi maviri, kukuvara kusingadzoreki kunogona kuitika.

Parizvino, iyo inonyanya kushandiswa DO sensors inogona chete kuyera yakaenzana mhepo saturation, pane mhedziso kusangana kweokisijeni yakanyungudika. Mushure mekuita sterilization yetsika yepakati, aeration uye kubvongodza kunoitwa kusvikira iyo sensor kuverenga yagadzikana, panguva iyo kukosha kunoiswa ku100% kuzara kwemhepo. Zviyero zvinotevera panguva yekuvirisa zvinoenderana nereferensi iyi. Mhedziso yeDO maitiro haigone kutariswa uchishandisa yakajairwa masensa uye inoda hunyanzvi hwepamberi, senge polarography. Nekudaro, zviyero zvekuzara kwemhepo zvinowanzokwana pakutarisa nekudzora maitiro ekuvirisa.

Mukati mekuvirisa, DO mazinga anogona kusiyana munzvimbo dzakasiyana. Kunyange kana kuverenga kwakatsiga kunowanikwa pane imwe nguva, kuchinja-chinja kungangoitika mune mamwe mapepanhau etsika. Maviri mahombe anowanzo ratidza kusiyana kwakakura kwenzvimbo mumazinga eDO, ayo anogona kukanganisa zvakanyanya kukura kwehutachiona uye kugadzirwa. Humbowo hwekuyedza hwakaratidza kuti, kunyangwe avhareji yeDO level inogona kunge iri 30%, kuita kwekuvirisa pasi pemamiriro ekuchinja kwakadzikira zvakanyanya pane pasi pemamiriro akagadzikana. Naizvozvo, mukuyera-kwepamusoro kwezvinovirisa-kupfuura kufunga kwejometri uye kufanana kwesimba-kuderedza misiyano yeDO yenzvimbo inoramba iri chinangwa chikuru chekutsvagisa.

Sei Yakanyungudika Oxygen Monitoring Yakakosha muBiopharmaceutical Fermentation?

1. Kuchengetedza Yakanyanya Kukura Kwenzvimbo yeMicroorganisms kana Masero
Kuviriswa kwemaindasitiri kunowanzo sanganisira aerobic microorganisms, senge Escherichia coli uye mbiriso, kana maseru anoyamwisa, akadai seChinese Hamster Ovary (CHO) maseru. Masero aya anoshanda se "vashandi" mukati meiyo fermentation system, inoda oxygen yekufema uye kuita metabolic. Okisijeni inoshanda seyekupedzisira maerekitironi kugamuchira mu aerobic kufema, ichigonesa kugadzirwa kwesimba muchimiro cheATP. Kushaikwa kweokisijeni yakakwana kunogona kukonzera kuvharika kwemasero, kusungwa kwekukura, kana kufa kwesero, zvichizoguma nekutadza kuvirisa. Kutarisisa DO mazinga anovimbisa kuti huwandu hweokisijeni hunoramba huri mukati meiyo yakakwana huwandu hwekusimudzira sero kukura uye kushanda.

2. Kuti Ive Nechokwadi Kushanda Synthesis yeTarget Products
Chinangwa chebiopharmaceutical fermentation hachisi chekusimudzira kuwanda kwesero chete asi kuti kufambisa kuumbwa kwezvigadzirwa zvinodikanwa, se insulin, monoclonal antibodies, vaccines, uye ma enzymes. Idzi nzira dzebiosynthetic dzinowanzoda simba rakakura rekuisa, rinobva pakufema kweaerobic. Pamusoro pezvo, akawanda enzymatic masisitimu anosanganisirwa muchigadzirwa synthesis anoenderana neokisijeni. Kushaikwa kweokisijeni kunogona kukanganisa kana kuderedza kushanda kwenzira idzi.

Uyezve, DO mazinga anoita sechiratidzo chekudzora. Zvese zviri zviviri zvakanyanyisa uye zvakaderera DO zvimiro zvinogona:
- Shandura nzira dzemagetsi emagetsi, semuenzaniso, kuchinjika kubva pakufema kweaerobic kuenda kune kushomeka kweanaerobic fermentation.
-Kukonzeresa cellular kushushikana mhinduro, zvichitungamira kugadzirwa kwezvinhu zvisingadiwe ne-zvigadzirwa.
- Kupesvedzera mazinga ekutaura eexogenous mapuroteni.

Nekunyatso kudzora mazinga eDO pamatanho akasiyana ekuvirisa, zvinogoneka kutungamira cellular metabolism kuenda kune yakanyanya kunangwa chigadzirwa synthesis, nekudaro uchiwana kuvirisa kwepamusoro uye goho repamusoro.

Nhanganyaya yeKuyerera Kwemvura Chati yeBio Pharmaceuticals

3. Kudzivirira Kushaikwa Kweokisijeni kana Kuwedzera
Kushaikwa kweokisijeni (hypoxia) inogona kuva nemhedzisiro yakaoma:
-Kukura kwesero uye kugadzirwa kwechigadzirwa kunomira.
- Metabolism inochinja kune anaerobic nzira, zvichikonzera kuunganidzwa kweasidhi organic senge lactic acid uye acetic acid, iyo inodzikisa pH yetsika yepakati uye inogona kuchetura maseru.
-Kurebesa hypoxia kunogona kukonzera kukuvara kusingadzoreki, nekupora kunenge kusina kukwana kunyangwe mushure mekudzorerwa kweokisijeni.

Okisijeni yakawandisa (supersaturation) inoisawo njodzi:
- Inogona kuunza oxidative kusagadzikana uye kuumbwa kweiyo reactive oxygen marudzi (ROS), iyo inokuvadza cell membranes uye biomolecules.
- Yakawandisa aeration uye kubvongodza kunowedzera mashandisiro esimba uye mutengo wekushandisa, zvichitungamira kune zvisina kufanira zviwanikwa tsvina.

4. SeCritical Parameter Yechokwadi-Nguva Monitoring uye Feedback Control

DO inguva chaiyo-nguva, inoenderera, uye yakazara paramende inoratidza mamiriro emukati meiyo yekuvirisa system. Shanduko mumatanho eDO anogona kuratidza zvine hunyoro dzakasiyana siyana dzemuviri uye dzekushanda:
-Kukura kwesero nekukurumidza kunowedzera kushandiswa kweokisijeni, zvichiita kuti DO mazinga adzike.
- Substrate kupera kana inhibition inononoka metabolism, kuderedza kushandiswa kweokisijeni uye kuita kuti DO mazinga asimuke.
-Kusvibiswa nehutachiona hwekunze kunoshandura maitiro ekushandisa okisijeni, zvichitungamira kune zvisina kujairika DO kuchinjika uye kushanda sechiratidzo chekutanga chekunyevera.
-Kusashanda zvakanaka kwezvishandiso, senge kutadza kukonzeresa, kuvharika kwepombi yemhepo, kana kusvibisa mafirita, zvinogonawo kukonzera hunhu husina kujairika hweDO.

Nekubatanidza chaiyo-nguva yeDO yekutarisa mune otomatiki yekudzora mhinduro sisitimu, kunyatso kudzora kwemazinga eDO kunogona kuwanikwa kuburikidza nekuchinja kune simba kweanotevera paramita:
-Kumhanyisa kumhanya: Kuwedzera kumhanya kunowedzera gasi-mvura yekubata nekupwanya mabhuru, nekudaro kunatsiridza kufambisa kweokisijeni. Iyi ndiyo nzira inonyanya kushandiswa uye inoshanda.
-Aeration rate: Kugadzirisa kuyerera kana kuumbwa kweiyo inlet gasi (semuenzaniso, kuwedzera chikamu chemhepo kana yakachena oxygen).
- Tangi kudzvanywa: Kusimudza kudzvanywa kunowedzera oksijeni chikamu chekumanikidza, nekudaro ichiwedzera solubility.
- Tembiricha: Kudzikisa tembiricha kunowedzera kunyungudika kweokisijeni mutsika yepakati.

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